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DMEM is a widely used basal medium for supporting the growth of many different mammalian cells.
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货品编号: Invitrogen.11885-084
品牌: Invitrogen
品名: DMEM, Low Glucose, Pyruvate
规格: 500ml/瓶
产品描述
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
内容及储存
Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
产品规格 Glutamine:L-Glutamine Phenol Red Indicator:Phenol Red Form:Liquid Glucose:Low Glucose Serum Supplementation:Standard Serum Supplementation HEPES Buffer:No HEPES Sodium Pyruvate Additive:Sodium Pyruvate Product Size:500 mL Volume (Metric):500 ml Green Features:Fewer resources used, Less waste Shipping Condition:Room Temperature
This DMEM is modified as follows: With Without • Low Glucose • HEPES • L-Glutamine • Sodium Pyruvate • Phenol Red The complete formulation is available. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
cGMP Manufacturing and Quality System Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM product made in our Scotland facility (31885-023). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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