货品编号: abcam.ab131366
品牌: abcam
品名: VeriBlot for IP Detection试剂(HRP) (ab131366)
规格: 500ul
产品描述:
VeriBlot for IP Detection Reagents are immunoblotting reagents that enable the trouble-free detection of
immunoblotted target protein bands, without interference from denatured IgG. This allows to detect the
(co-)immunoprecipitated protein without masking by the IgG heavy (50 kDa) and light chains (25 kDa).
In general, this interference tends to originate from secondary antibodies which recognize primary antibodies
released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate
itself. VeriBlot for IP detection reagents only recognize native (non-reduced) antibodies and therefore the
detection of heavy and light chains is highly minimized, if the immunoprecipitate is fully reduced.