品牌: Invitrogen
品名: Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488
规格: 1MG
产品描述:
To minimize cross-reactivity, the goat anti-rabbit IgG whole antibodies have been pre cross-adsorbed against bovine IgG,
goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity
of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a
column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding
secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through
additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are
apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or
in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.