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Catalog number: HRP-60008
Size: 50 μg/100 μl
Source: Mouse
Isotype: IgM
Synonyms: ACTB; ACTB, Acn, acn, beta, Acn, cytoplasmic 1, B acn, Beta acn, F acn, HRP β acn, HRP-ACTB, HRP-Beta acn, HRP-F acn, PS1TP5BP1, β acn
Applications:
Tested applicaons: ELISA, WB
Species specificity: Human, Mouse, Rat, Zebra Fish; other species not tested.
Caculated beta Actin MW: 375aa,42 kDa
Observed beta Actin MW: 42 kDa
Posive WB detected in : HeLa cells
Recommended diluon: WB: 1:2000-1:20000
Background:
Beta acn, also named as ACTB and F-Acn, belongs to the acn family. Acns are highly conserved globular proteins that are involved in various types of cell molity and are ubiquitously expressed in all eukaryoc cells. At least six isoforms of acns are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aorc smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle acn proteins. Most acns consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta acn has been widely used as the internal control in RT-PCR and Western Blo&ng as a 42- kDa protein. This anbody can recognize all of acns. This anbody is conjugated with HRP.Note: Do not add Azium (Sodium Azide or Smite) into the diluon buffer. Azium is the HRP inhibitor which decreases the enzymac acvity of HRP.Immunogen information:
Immunogen: Recombinant Protein
GenBank accession number: BC002409
Gene ID (NCBI): 60
Full name: Actin, beta
Product information
Purificaon method: Caprylic acid/ammonium sulfate precipitaon
Storage: PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20 oC.
Western blot analysis of beta acn in various ssues and cell lines using Proteintech anbody HRP-60008 at a diluon of 1:5000.