Thermo.S0015 Gibco Human Keratinocyte Growth Supplement (HKGS) 5ML

     Medium 154 to culture human epidermal keratinocytes.

     HKGS is an ionically-balanced supplement containing bovine pituitary extract (BPE), recombinant human insulin-like growth factor-I, 

     hydrocortisone, bovine transferrin, and human epidermal growth factor. Each 5-mL bottle of HKGS contains all of the growth factors, 

     hormones, and tissue extracts necessary for the culture of human epidermal keratinocytes and is the correct amount of supplement 

     for a 500-mL bottle of medium.

     When a 500-mL bottle of medium is supplemented with HKGS, the final concentrations of the components in the supplemented 

     medium are:

     • Bovine pituitary extract (BPE): 0.2% v/v
     • Recombinant human insulin-like growth factor-I: 0.01 µg/mL   
     • Hydrocortisone: 0.18 µg/mL
     • Bovine transferrin: 5 µg/mL
     • Human epidermal growth factor: 0.2 ng/mL