货品编号: Invitrogen.10565018
品牌: Invitrogen
品名: DMEM/F-12, GlutaMAX™ supplement
规格: 500ml
产品描述
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting
the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells,
fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of
cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
With Without
• GlutaMAX™ • HEPES
• Phenol Red
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose,
amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX™ supplement minimizes
toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins,
lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS).
DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA
as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12
product made in our Scotland facility (31331-028). This facility is also registered with the FDA as a medical device manufacturer and
certified to the ISO 13485 standard.